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Volume 48S Number 2

Volume 48S Number 2

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Methods for diagnosing diseases of the uterine cervix

Nilufar O. Navruzova^a*, Elnora E. Karshiyeva^b, Gulchekhra A. Ikhtiyarova^a

 
ABSTRACT:     The purpose of the study was - the study the state of the vaginal microbiocenosis and determination of the presence of diseases of the cervix, using the PCR method (Femoflor-16) - for the differential diagnosis of dysbiotic disorders and infectious and inflammatory diseases of genitalia in women. Clinical and laboratory examination of 105 patients with cervical diseases was carried out. All patients underwent the necessary diagnostic standard: an examination of the cervix in the mirrors, cytological examination of smears from the ecto- and endocervix, polymerase chain reaction (PCR) for HPV, pH-metry, extended colposcopy, histological examination of cervical biopsies. Material for the study of vaginal microbiocenosis was collected from the posterolateral wall of the vagina, for HPV detection - from the cervical canal. The scraping was placed in an Eppendorf tube containing 1 ml of saline; storage and transportation of the material was carried out in accordance with the current regulatory documents. DNK was isolated using a PROBA-GS reagent kit (Standard Diagnostics, Bukhara). The study was carried out by PCR with detection of results in real time (RT-PCR) using Femoflor-16 reagents (Standard Diagnostics, Bukhara) in a detecting amplifier DT-96, according to the manufacturer's instructions, in the laboratory (Standard Diagnostics, g . Bukhara). In our study, in women with complaints of minor and moderate vaginal discharge - 41.7%, severe vaginal discharge disturbed 7.8% of patients. Complaints of itching and or burning were reported by 33% of patients, vaginal discomfort - 30%, urination disorder - 15.5%, pain during intercourse - 11.6%. The variety of leading pathogens dictates the need for a comprehensive study of the vaginal microbiocenosis in patients with precancerous pathology of the cervix, which allows, with high accuracy and specificity, to give a quantitative and qualitative assessment of the main participants in the studied biotope for the purpose of individual therapy.

* Corresponding author, E-mail:

Received 30 Aug 2022, Accepted 22 Nov 2022